Taylor K, Howard CB, Jones ML, Sedliarou I, MacDiarmid J, Brahmbhatt H et al (2015) Nanocell targeting using engineered bispecific antibodies. Mabs 7(1):53–65 Tustian AD, Endicott C, Adams B, Mattila J, Bak H (2016) Development of purification processes for fully human bispecific antibodies based upon modification of protein A binding avidity. Mabs 8(4):828–838

Skegro D, Stutz C, Ollier R, Svensson E, Wassmann P, Bourquin F et al (2017) Immunoglobulin domain interface exchange as a platform technology for the generation of Fc heterodimers and bispecific antibodies. J Biol Chem 292(23):9745–9759 Oldenburg J, Mahlangu JN, Kim B, Schmitt C, Callaghan MU, Young G et al (2017) Emicizumab prophylaxis in hemophilia A with inhibitors. N Engl J Med 377(9):809–818 Cleaning brass door handles is a great way to refresh the look of a room instantly, but traditional polishing methods can be time-consuming and require frequent applications. Fortunately, several shortcuts make the process simpler. For instance, you can mix two parts white vinegar with one part salt in the water to create a cleaning solution. Rub the solution onto the brass handle with a soft cloth for effective results. If the grime on the handle hasn’t completely gone, simply rub an almond oil-wrapped cloth into the handle’s surface until all signs of dirt are gone.Restore the shine of your brass door knobs and handles using the brass polish you bought from the store. Step 1: Wipe with Soap and Water. Geuijen CAW, Kruif CAD, Throsby M, Logtenberg T, Berthold A, Bakker H. (2015). Antibody that binds erbb-2 and erbb-3. WO2015130173 A1, 3 Sep 2015. The optimal loading conditions for Capto S ImpAct was evaluated in the bind and elute mode by a screening study between pH 5.0–7.5 and 0–100 mM NaCl. The highest SBC was obtained at low pH, with 0–100 mM NaCl having no difference for FabscFv-KiH and no NaCl preferred for Fab 2scFv-KiH. The optimal loading condition was therefore determined to be pH 5.0, with no salt concentration for both molecules. The maximum SBC determined in this way is 58 g/L-R or 45 g/L-R for FabscFv-KiH and Fab 2scFv-KiH, respectively (Fig. 3e, f). Applying metal polish to brass door handles is a simple way to keep them looking their best. Before applying the metal polish, ensure the handles are clean and dry. Start using a toothbrush or soft cloth to dust away dirt and debris, taking extra care around crevices and hard-to-reach spots. Once the handle’s surface is clean, you can use a small amount of metal polish to apply it in a circular motion over the entire handle. Allow enough time for the polish to sit before wiping it off with a damp cloth to draw out the remaining dirt from any little nooks and crannies. Finally, buff up the handle with a dry cloth until it has a lovely sheen that will last for some time before regularly repeating this process. Applying a protective coating One of the most tarnish-affected materials on earth, you can remove silver tarnish with a variety of ready-made store bought products. However, if you want to save some cash and time, you might consider this next solution, which would require the temporary removal of some of your hardware.

Oh, and if you don’t have WD-40, some of my online friends recommend Greased Lightening. I’ve never used this product, so I can’t give it a thumbs up or down. If you use it, I’m love to hear your thoughts in the comments.The use of steel wool and steel brushes can leave particles on surfaces which can lead to rusting or corrosion – these should be avoided. You likely use them every day without giving their cleanliness a passing thought, even when you’re doing a thorough spring cleaning of your home. They are building up dirt, grime, and invisible bacteria with each use. That’s right, we’re talking about door knobs. Kufer P, Raurn T, Hoffmann P, Kischel R, Lutterbuese R, Rau D et al. (2014). Binding molecules for bcma and cd3. WO2014140248 A1, 18 Sep 2014. For both stability and 96-well plate study, post-Protein A eluate (pH 6, 50 mM Na-citrate, no salt) was concentrated to 20 mg/mL using Amicon Ultra 15-mL filters (50 kDa NMWL), followed by adjustments to the required bsAb, pH and salt concentration. Dip a clean sponge or cloth into the liquid, use it to clean your door hardware, and wipe the soap away with a fresh, damp cloth. Finish by polishing exterior bronze door hardware with a soft cloth for a seamless finish. Cleaning My Aged Bronze Door Handle with Ketchup

Although the HMW species was quantitatively very similar in both FabscFv-KiH and Fab 2scFv-KiH post-Protein A eluates at ~ 6%, ~ 2.5% of HMW species was obtained when 120 mg/mL-R and 60 mg/mL-R of FabscFv-KiH and Fab 2scFv-KiH post-Protein A eluates were loaded onto Capto Butyl ImpRes, respectively. The fact that the same relative percentage of HMW species can be yielded at double the load of FabscFv-KiH post-Protein A eluate compared to that of Fab 2scFv-KiH reflects a higher binding capability of Capto Butyl ImpRes towards the HMW species present in the former compared to the latter. This highlights the importance of investigating the removal of different species as a function of different loading amounts, as the type and physicochemical properties of the exact species present in the sample, in addition to the quantity, plays an important role in determining the effectiveness of their removal. One of the most important reasons why you should clean your stove knobs regularly is to keep your kitchen area neat and organized. Stove knobs can easily accumulate dirt, grease, grime, and oil from frequent cooking activities, which can make the area look cluttered and messy. Regularly cleaning stove knobs will help keep your kitchen looking presentable. 2. To Reduce the Risk of Bacterial Contamination If your hardware has a highly glossed finish, your attempts to clean the subsurface material will fail. To remove clear coats and get to the nitty gritty, you’ll want to use mineral spirits or another solvent. You can always reapply the clear coat after the cleaning process. Two Easy At-Home Cleaning Solutions for Brass and Copper Door Knobs Recipe #1: Dorken B, Riethmuller G, Kufer P, Lutterbuse R, Bargou R, Loffler A. (2009). CD19XCD3 specific polypeptides and uses thereof. US7575923 B2, 18 Aug 2009. Smith EJ, Olson K, Haber LJ, Varghese B, Duramad P, Tustian AD et al (2015) A novel, native-format bispecific antibody triggering T-cell killing of B-cells is robustly active in mouse tumor models and cynomolgus monkeys. Sci Rep 5:17943Sometimes, all you need is mild dish soap to get a door knob clean, while other times, it may be necessary to use a bleach solution to kill germs and bacteria. Learn how to use various cleaners for different handles to leave them shiny and clean. Cleaning Door Knobs Made of Different Materials After you have cleaned your stainless steel surface, immediately rinse with hot water and dry with a clean cloth or towel.

Michaelson JS, Demarest SJ, Miller B, Amatucci A, Snyder WB, Wu X et al (2009) Anti-tumor activity of stability-engineered IgG-like bispecific antibodies targeting TRAIL-R2 and LTbetaR. Mabs 1(2):128–141 Manzke O, Tesch H, Diehl V, Bohlen H (1997) Single-step purification of bispecific monoclonal antibodies for immunotherapeutic use by hydrophobic interaction chromatography. J Immunol Methods 208(1):65–73 Hall T, Wilson JJ, Brownlee TJ, Swartling JR, Langan SE, Lambooy PK (2015) Alkaline cation-exchange chromatography for the reduction of aggregate and a mis-formed disulfide variant in a bispecific antibody purification process. J Chromatogra b: Analyt Technol Biomed Life Sci 975:1–8Modern brass is usually lacquered with a clear finish to stop it discolouring and by comparison to old brass looks yellowish in colour. Cleaning modern brass is easy, simply wipe with a soft damp cloth. If it’s really grimy, use a mild mix of washing up liquid and warm water. Wipe the surface clean, wipe with a clean cloth and dry thoroughly. Brass cleaning home-remedies If your doorknobs are made of brass, I think it would be most accessible and practical to clean brass with vinegar. And in this way, you can save yourself the cash or pressure of consistently hitting the store for a brass cleaning solution. Brischwein K, Schlereth B, Guller B, Steiger C, Wolf A, Lutterbuese R et al (2006) MT110: a novel bispecific single-chain antibody construct with high efficacy in eradicating established tumors. Mol Immunol 43(8):1129–1143 For stubborn stains or dirt, use a mild, non-scratching abrasive powder such as typical household cleaners. A more aggressive form of sponge, cloth, or bristled brush can be used here. However, steel wool and carbon steel brushes should be avoided, as they can leave particles embedded in your stainless steel surfaces, leading to rusting over time. Note: If You Want To Learn More Know About How To Clean A Brass Chandelier How To Clean Brass Door Knobs and Polish